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KMID : 0545120080180040725
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 4 p.725 ~ p.729
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Production of Cyclodextrins in Ultrafiltration Membrane Reactor Containing Cyclodextrin Glycosyltransferase from Bacillus macerans
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Son Young-Jin
Rha Chan-Su
Park Yong-Cheol
Shin So-Yeon
Lee Yoon-Seung
Seo Jin-Ho
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Abstract
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An enzyme reactor installed with ultrafiltration membrane was developed to produce α-, β-, and γ-cyclodextrins (CDs) from soluble starch by Bacillus macerans cyclodextrin glycosyltransferase (CGTase) tagged with 10 lysines at its C-terminus (CGTK10ase). Ultrafiltration membrane YM10 with 10,000 of molecular cutoff was chosen for membrane modification and CD production. A repeated-batch type of the enzyme reaction with free CGTK10ase resulted in a α-CD yield of 24.0 (±1.5)% and a productivity of 4.68 (±0.88) g/l-h, which were 7 times higher that those for CGTK10ase immobilized on modified YM10 membrane. Addition of 1- nonanol increased CD yields by 30% relative to the control, which might be due to prevention of the reversible hydrolysis of CDs.
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KEYWORD
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Cyclodextrin glycosyltransferase, immobilization, ultrafiltration membrane, enzyme reactor, α-cyclodextrin
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